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ProductsBack/GRIK1 / GLUR5 Antibody

GRIK1 / GLUR5 Antibody

LS-C497183
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetGRIK1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GRIK1 / GLUR5 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 102kDa/103kDa, while the observed MW by Western blot was Refer to Figures.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 102kDa/103kDa, while the observed MW by Western blot was Refer to Figures.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2897
  • Target name
    GRIK1
  • Target description
    glutamate ionotropic receptor kainate type subunit 1
  • Target synonyms
    EAA3, EEA3, GLR5, GLUR5, GluK1, gluR-5, glutamate receptor ionotropic, kainate 1, GluK1(del_e2), excitatory amino acid receptor 3, glutamate receptor 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of GRIK1 using anti-GRIK1 antibody (PB9208). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human GRIK1 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIK1 antigen affinity purified polyclonal antibody (Catalog # PB9208) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIK1 at approximately 40 kDa. The expected band size for GRIK1 is at 40 kDa.
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