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Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.

Anti-GRM2 Antibody

HPA065166
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGRM2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GRM2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2912
  • Target name
    GRM2
  • Target description
    glutamate metabotropic receptor 2
  • Target synonyms
    GLUR2, GPRC1B, MGLUR2, mGlu2, metabotropic glutamate receptor 2, glutamate receptor homolog, glutamate receptor, metabotropic 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14416
  • Protein Name
    Metabotropic glutamate receptor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of GRM2 using anti-GRM2 antibody (A06123-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM2 antigen affinity purified polyclonal antibody (Catalog # A06123-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRM2 at approximately 100kDa and >200 kDa. The expected band size for GRM2 is at 96 kDa.
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