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Western blot analysis in human cell lines U2OS and HEK293 using Anti-GSDMD antibody. Corresponding GSDMD RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Western blot analysis in human cell lines U2OS and HEK293 using Anti-GSDMD antibody. Corresponding GSDMD RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Western blot analysis in human cell lines U2OS and HEK293 using Anti-GSDMD antibody. Corresponding GSDMD RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Anti-GSDMD Antibody

HPA044487
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetGSDMD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GSDMD
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79792
  • Target name
    GSDMD
  • Target description
    gasdermin D
  • Target synonyms
    DF5L, DFNA5L, FKSG10, GSDMDC1, gasdermin-D, gasdermin domain containing 1, gasdermin domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP57764
  • Protein Name
    Gasdermin-D
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of GSDMD using anti-GSDMD antibody (A02842). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human THP-1(+TPA+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSDMD antigen affinity purified polyclonal antibody (Catalog # A02842) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSDMD at approximately 30, 53 kDa. The expected band size for GSDMD is at 53 kDa.
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