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GSDMD antibody

70R-17613
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    GSDMD antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western blot All lanes: GSDMD antibody at 4microg/ml Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A431 whole cell lysate Lane 4: U251 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
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Western blot analysis in human cell lines U2OS and HEK293 using Anti-GSDMD antibody. Corresponding GSDMD RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
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Figure 1. Western blot analysis of GSDMD using anti-GSDMD antibody (A02842). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human THP-1(+TPA+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSDMD antigen affinity purified polyclonal antibody (Catalog # A02842) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSDMD at approximately 30, 53 kDa. The expected band size for GSDMD is at 53 kDa.
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