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Anti-GSPT1 Antibody

144-64210
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetGSPT1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GSPT1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID2935
  • Target name
    GSPT1
  • Target description
    G1 to S phase transition 1
  • Target synonyms
    551G9.2, ETF3A, GST1, eRF3a, eukaryotic peptide chain release factor GTP-binding subunit ERF3A, G1 to S phase transition protein 1 homolog, eukaryotic peptide chain release factor subunit 3a, eukaryotic release factor 3a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15170
  • Protein Name
    Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
  • Scientific Description
    GSPT1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GSPT1,2 using anti-GSPT1,2 antibody (A07761-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSPT1,2 antigen affinity purified polyclonal antibody (Catalog # A07761-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSPT1,2 at approximately 85 kDa. The expected band size for GSPT1,2 is at 85 kDa.
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