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Immunohistochemical staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.

Anti-GSPT1 Antibody

HPA052488
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetGSPT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GSPT1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2935
  • Target name
    GSPT1
  • Target description
    G1 to S phase transition 1
  • Target synonyms
    551G9.2; eRF3a; ETF3A; eukaryotic peptide chain release factor GTP-binding subunit ERF3A; eukaryotic peptide chain release factor subunit 3a; eukaryotic release factor 3a; G1 to S phase transition protein 1 homolog; GST1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15170
  • Protein Name
    Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GSPT1,2 using anti-GSPT1,2 antibody (A07761-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSPT1,2 antigen affinity purified polyclonal antibody (Catalog # A07761-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSPT1,2 at approximately 85 kDa. The expected band size for GSPT1,2 is at 85 kDa.
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