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Anti-GSTP1 [SAIC-22D-22]

AB00312-1.1-BT
Absolute Antibody
ApplicationsMass Spectrometry
Product group Antibodies
TargetGSTP1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-GSTP1 [SAIC-22D-22]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Original data characterizing this antibody may be found here .
  • Applications
    Mass Spectrometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAIC-22D-22
  • Gene ID2950
  • Target name
    GSTP1
  • Target description
    glutathione S-transferase pi 1
  • Target synonyms
    deafness, X-linked 7; DFN7; epididymis secretory protein Li 22; FAEES3; fatty acid ethyl ester synthase III; glutathione S-transferase P; GST class-pi; GST3; GSTP; GSTP1-1; HEL-S-22; PI
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09211
  • Protein Name
    Glutathione S-transferase P
  • Scientific Description
    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3/GST pi antigen affinity purified polyclonal antibody (Catalog # PB9184) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST3/GST pi at approximately 23 kDa. The expected band size for GST3/GST pi is at 23 kDa.
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Western blot All lanes: GSTP1 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 24 kDa Observed band size: 30 kDa
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