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ProductsBack/GSTP1 / GST Pi Antibody (clone 1D2-F8-G10, Preservative Free)

GSTP1 / GST Pi Antibody (clone 1D2-F8-G10, Preservative Free)

LS-C743671
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGSTP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GSTP1 / GST Pi Antibody (clone 1D2-F8-G10, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:320000), WB (1:1000 - 1:5000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D2-F8-G10
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2950
  • Target name
    GSTP1
  • Target description
    glutathione S-transferase pi 1
  • Target synonyms
    DFN7, FAEES3, GST3, GSTP, HEL-S-22, PI, glutathione S-transferase P, GST class-pi, GSTP1-1, deafness, X-linked 7, epididymis secretory protein Li 22, fatty acid ethyl ester synthase III
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3/GST pi antigen affinity purified polyclonal antibody (Catalog # PB9184) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST3/GST pi at approximately 23 kDa. The expected band size for GST3/GST pi is at 23 kDa.
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