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Anti-GZMA Antibody

144-06231
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetGZMA
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GZMA Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3001
  • Target name
    GZMA
  • Target description
    granzyme A
  • Target synonyms
    CTL tryptase; CTLA3; cytotoxic T-lymphocyte proteinase 1; Cytotoxic T-lymphocyte-associated serine esterase-3; fragmentin-1; granzyme 1; granzyme A; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease); granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); h factor; Hanukah factor serine protease); HF; HFSP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12544
  • Protein Name
    Granzyme A
  • Scientific Description
    GZMA Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Granzyme A/GZMA using anti-Granzyme A/GZMA antibody (A02779-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Granzyme A/GZMA antigen affinity purified polyclonal antibody (Catalog # A02779-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Granzyme A/GZMA at approximately 29KD. The expected band size for Granzyme A/GZMA is at 29KD.
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