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ProductsBack/Anti-human Granzyme A mAb (GA29), unconjugated
Anti-human Granzyme A mAb (GA29), unconjugated

Anti-human Granzyme A mAb (GA29), unconjugated

3484-3
Mabtech
ApplicationsELISA
Product group Antibodies
TargetGZMA
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human Granzyme A mAb (GA29), unconjugated
  • Delivery Days Customer
    9
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GA29
  • Conjugate
    Unconjugated
  • Gene ID3001
  • Target name
    GZMA
  • Target description
    granzyme A
  • Target synonyms
    CTLA3, HFSP, granzyme A, CTL tryptase, Cytotoxic T-lymphocyte-associated serine esterase-3, Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease), HF, Hanukah factor serine protease), cytotoxic T-lymphocyte proteinase 1, fragmentin-1, granzyme 1, granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3), h factor
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of human Granzyme A in immunoassays such as ELISA.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Granzyme A/GZMA using anti-Granzyme A/GZMA antibody (A02779-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Granzyme A/GZMA antigen affinity purified polyclonal antibody (Catalog # A02779-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Granzyme A/GZMA at approximately 29KD. The expected band size for Granzyme A/GZMA is at 29KD.
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