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Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.

Anti-H1FNT Antibody

HPA046204
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetH1-7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-H1FNT Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID341567
  • Target name
    H1-7
  • Target description
    H1.7 linker histone
  • Target synonyms
    H1.7, H1FNT, H1T2, testis-specific H1 histone, H1 histone family member N, testis specific, HANP1, haploid germ cell-specific nuclear protein 1, histone H1.7, histone H1t2, testis secretory sperm-binding protein Li 223n
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ75WM6
  • Protein Name
    Testis-specific H1 histone
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of H1-7 using anti-H1-7 antibody (A13834-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse testis tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H1-7 antigen affinity purified polyclonal antibody (Catalog # A13834-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H1-7 at approximately 28 kDa. The expected band size for H1-7 is at 28 kDa.
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