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ProductsBack/H1FNT Antibody (aa59-93)

H1FNT Antibody (aa59-93)

LS-C392904
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetH1-7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    H1FNT Antibody (aa59-93)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Peptide-ELISA, WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID341567
  • Target name
    H1-7
  • Target description
    H1.7 linker histone
  • Target synonyms
    H1.7, H1FNT, H1T2, testis-specific H1 histone, H1 histone family member N, testis specific, HANP1, haploid germ cell-specific nuclear protein 1, histone H1.7, histone H1t2, testis secretory sperm-binding protein Li 223n
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of H1-7 using anti-H1-7 antibody (A13834-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse testis tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H1-7 antigen affinity purified polyclonal antibody (Catalog # A13834-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H1-7 at approximately 28 kDa. The expected band size for H1-7 is at 28 kDa.
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