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Immunohistochemical staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.

Anti-HADHA Antibody

HPA015536
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHADHA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HADHA
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3030
  • Target name
    HADHA
  • Target description
    hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
  • Target synonyms
    ECHA, GBP, HADH, LCEH, LCHAD, MLCL AT, MTPA, TP-ALPHA, trifunctional enzyme subunit alpha, mitochondrial, 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit, 3-oxoacyl-CoA thiolase, 78 kDa gastrin-binding protein, gastrin-binding protein, hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit, hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit, long-chain 2-enoyl-CoA hydratase, long-chain-3-hydroxyacyl-CoA dehydrogenase, mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit, mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alpha subunit, mitochondrial trifunctional enzyme, alpha subunit, mitochondrial trifunctional protein, alpha subunit, monolysocardiolipin acyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40939
  • Protein Name
    Trifunctional enzyme subunit alpha, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HADHA using anti-HADHA antibody (A03666-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHA antigen affinity purified polyclonal antibody (Catalog # A03666-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for HADHA at approximately 79 kDa. The expected band size for HADHA is at 83 kDa. Lane 1: human 293T whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHA antigen affinity purified polyclonal antibody (Catalog # A03666-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HADHA at approximately 79 kDa. The expected band size for HADHA is at 83 kDa.
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Lane 1: 293T cell lysates; Lane 2: HADHA knockout (KO) 293T cell lysates probed with HADHA Polyclonal Antibody, Unconjugated (bs-55092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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