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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.

Anti-HCN1 Antibody

HPA019195
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHCN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HCN1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID348980
  • Target name
    HCN1
  • Target description
    hyperpolarization activated cyclic nucleotide gated potassium channel 1
  • Target synonyms
    BCNG-1, BCNG1, DEE24, EIEE24, GEFSP10, HAC-2, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, MUT-D534H potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, MUT-L400P potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, MUT-M243delinsTL potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, brain cyclic nucleotide-gated channel 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60741
  • Protein Name
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of HCN1 using anti-HCN1 antibody (PB9210). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCN1 antigen affinity purified polyclonal antibody (Catalog # PB9210) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCN1 at approximately 120 kDa. The expected band size for HCN1 is at 99 kDa.
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IHC image of CSB-PA010216LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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