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HCN1 antibody

GTX131334
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHCN1
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Overview

  • Supplier
    GeneTex
  • Product Name
    HCN1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.45 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID348980
  • Target name
    HCN1
  • Target description
    hyperpolarization activated cyclic nucleotide gated potassium channel 1
  • Target synonyms
    BCNG-1, BCNG1, DEE24, EIEE24, GEFSP10, HAC-2, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, MUT-D534H potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, MUT-L400P potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, MUT-M243delinsTL potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, brain cyclic nucleotide-gated channel 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60741
  • Protein Name
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Scientific Description
    Hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Monzón-Sandoval J, Poggiolini I, Ilmer T, et al. Human-Specific Transcriptome of Ventral and Dorsal Midbrain Dopamine Neurons. Ann Neurol. 2020,87(6):853-868. doi: 10.1002/ana.25719
    Read this paper

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Figure 1. Western blot analysis of HCN1 using anti-HCN1 antibody (PB9210). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCN1 antigen affinity purified polyclonal antibody (Catalog # PB9210) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCN1 at approximately 120 kDa. The expected band size for HCN1 is at 99 kDa.
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