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Immunohistochemical staining of human testis shows moderate to strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Anti-HENMT1 Antibody

HPA028464
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHENMT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HENMT1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID113802
  • Target name
    HENMT1
  • Target description
    HEN methyltransferase 1
  • Target synonyms
    C1orf59, HEN1, small RNA 2'-O-methyltransferase, HEN1 methyltransferase homolog 1, hua enhancer 1 homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5T8I9
  • Protein Name
    Small RNA 2'-O-methyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HENMT1 using anti-HENMT1 antibody (A14219-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HENMT1 antigen affinity purified polyclonal antibody (Catalog # A14219-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HENMT1 at approximately 45 kDa. The expected band size for HENMT1 is at 45 kDa.
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