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HENMT1 Antibody

LS-C830510
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHENMT1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HENMT1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:30 - 1:150), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID113802
  • Target name
    HENMT1
  • Target description
    HEN methyltransferase 1
  • Target synonyms
    C1orf59; HEN1; HEN1 methyltransferase homolog 1; hua enhancer 1 homolog 1; small RNA 2'-O-methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HENMT1 using anti-HENMT1 antibody (A14219-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HENMT1 antigen affinity purified polyclonal antibody (Catalog # A14219-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HENMT1 at approximately 45 kDa. The expected band size for HENMT1 is at 45 kDa.
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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HENMT1 Polyclonal Antibody, Unconjugated (bs-15454R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining
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Immunohistochemical staining of human testis shows moderate to strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.
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