Anti-Heparan Sulphate Proteoglycan [A7L6]

Absolute Antibody

Anti-Heparan Sulphate Proteoglycan [A7L6]

9

This antibody recognizes and precipitates perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. This macromolecule has the power to store and protect growth factors, and thus plays a major rol in the contro

This original IgG2a rat clone can be used to detect and analyze the presence of perlecan. The specificity towards this protein has been confirmed using immunoprecipitation (Couchman, Ljubimov, 1989; PMID: 2477673). The perlecan was confirmed to reside in the basement membrane by means of immunofluorescence using this antibody (Couchman, Ljubimov, 1989; PMID: 2477673). Immunohistochemical staining confirmed that this clone also cross-reacts with other mammalian creatures (Couchman, Ljubimov, 1989; PMID: 2477673). In addition, one group used western blotting and immunohistochemical staining to detect significant differences in weight and distribution of antibodies across heparan sulfate proteoglycans (Tingboe et al., 2006; PMID: 16459119).

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry

Research Use Only

Monoclonal

A7L6

HSPG2

heparan sulfate proteoglycan 2

basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); HSPG; perlecan proteoglycan; PLC; PRCAN; SJA; SJS; SJS1

Human

IgG1

Basement membrane-specific heparan sulfate proteoglycan core protein

This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Bovine, Fish, Human, Mouse, Rat

-20°C,2°C to 8°C

12352203