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ProductsBack/Anti-Heparanase 1 Monoclonal Antibody
Western blot analysis of Heparanase 1 expression in K562 cell lysate.
Western blot analysis of Heparanase 1 expression in K562 cell lysate.
Western blot analysis of Heparanase 1 expression in K562 cell lysate.

Anti-Heparanase 1 Monoclonal Antibody

M01313
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHPSE
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Heparanase 1 Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AFOF-8
  • Gene ID10855
  • Target name
    HPSE
  • Target description
    heparanase
  • Target synonyms
    HPA, HPA1, HPR1, HPSE1, HSE1, heparanase, endo-glucoronidase, heparanase-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y251
  • Protein Name
    Heparanase
  • Scientific Description
    Boster Bio Anti-Heparanase 1 HPSE Monoclonal Antibody catalog # M01313. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ye TS, Zhang YW, Zhang XM. Protective effects of Danggui Buxue Tang on renal function, renal glomerular mesangium and heparanase expression in rats with streptozotocin-induced diabetes mellitus. Exp Ther Med. 2016,11(6):2477-2483.
    Read this paper
  • Tang D, Piao Y, Zhao S, et al. Expression and correlation of matrix metalloproteinase-9 and heparanase in patients with breast cancer. Med Oncol. 2014,31(7):26. doi: 10.1007/s12032-014-0026-4
    Read this paper
  • Tang D, Zhang Q, Zhao S, et al. The expression and clinical significance of microRNA-1258 and heparanase in human breast cancer. Clin Biochem. 2013,46(10-11):926-32. doi: 10.1016/j.clinbiochem.2013.01.027
    Read this paper
  • Ma ST, Liu DL, Deng JJ, et al. Effect of arctiin on glomerular filtration barrier damage in STZ-induced diabetic nephropathy rats. Phytother Res. 2013,27(10):1474-80. doi: 10.1002/ptr.4884
    Read this paper

Datasheet

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MSDS

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Figure 1. Western blot analysis of Heparanase 1 using anti-Heparanase 1 antibody (PB9427). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Human Placenta Tissue Lysate at 50ug, Lane 3: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heparanase 1 antigen affinity purified polyclonal antibody (Catalog # PB9427) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heparanase 1 at approximately 61 kDa. The expected band size for Heparanase 1 is at 61 kDa.
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