Figure 1. Western blot analysis of HIF-2-Alpha/EPAS1 using anti-HIF-2-Alpha/EPAS1 antibody (A01248-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2-Alpha/EPAS1 antigen affinity purified polyclonal antibody (Catalog # A01248-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF-2-Alpha/EPAS1 at approximately 96KD. The expected band size for HIF-2-Alpha/EPAS1 is at 96KD.