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Western Blot analysis of Jurkat L929 cells using EPAS-1 Polyclonal Antibody
Western Blot analysis of Jurkat L929 cells using EPAS-1 Polyclonal Antibody
Western Blot analysis of Jurkat L929 cells using EPAS-1 Polyclonal Antibody

EPAS1 Antibody

CSB-PA005478
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetEPAS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EPAS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2034
  • Target name
    EPAS1
  • Target description
    endothelial PAS domain protein 1
  • Target synonyms
    basic-helix-loop-helix-PAS protein MOP2; bHLHe73; class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99814
  • Protein Name
    Endothelial PAS domain-containing protein 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HIF-2-Alpha/EPAS1 using anti-HIF-2-Alpha/EPAS1 antibody (A01248-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2-Alpha/EPAS1 antigen affinity purified polyclonal antibody (Catalog # A01248-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF-2-Alpha/EPAS1 at approximately 96KD. The expected band size for HIF-2-Alpha/EPAS1 is at 96KD.
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