Figure 1. Western blot analysis of HLF using anti-HLF antibody (A01284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat lung whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLF antigen affinity purified polyclonal antibody (Catalog # A01284-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLF at approximately 33KD. The expected band size for HLF is at 33KD.
Figure 1. Western blot analysis of HLF using anti-HLF antibody (A01284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat lung whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLF antigen affinity purified polyclonal antibody (Catalog # A01284-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLF at approximately 33KD. The expected band size for HLF is at 33KD.
Anti-HLF Antibody Picoband(r)
A01284-1-HRP
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHLF
Overview
- SupplierBoster Bio
- Product NameAnti-HLF Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID3131
- Target nameHLF
- Target descriptionHLF transcription factor, PAR bZIP family member
- Target synonymsE2A-HLF fusion transcript protein; hepatic leukemia factor; HLF, PAR bZIP transcription factor; PAR bZIP transcription factor
- HostRabbit
- IsotypeIgG
- Protein IDQ16534
- Protein NameHepatic leukemia factor
- Scientific DescriptionBoster Bio Anti-HLF Antibody Picoband® catalog # A01284-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203