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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.

Anti-HLF Antibody

HPA068156
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHLF
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HLF
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3131
  • Target name
    HLF
  • Target description
    HLF transcription factor, PAR bZIP family member
  • Target synonyms
    E2A-HLF fusion transcript protein; hepatic leukemia factor; HLF, PAR bZIP transcription factor; PAR bZIP transcription factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16534
  • Protein Name
    Hepatic leukemia factor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HLF using anti-HLF antibody (A01284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat lung whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLF antigen affinity purified polyclonal antibody (Catalog # A01284-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLF at approximately 33KD. The expected band size for HLF is at 33KD.
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