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Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Anti-HLTF Antibody

HPA015284
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHLTF
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HLTF
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6596
  • Target name
    HLTF
  • Target description
    helicase like transcription factor
  • Target synonyms
    HIP116, HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3, ZBU1, helicase-like transcription factor, DNA-binding protein/plasminogen activator inhibitor-1 regulator, RING finger protein 80, RING-type E3 ubiquitin transferase HLTF, SNF2-like 3, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, sucrose nonfermenting protein 2-like 3, sucrose nonfermenting-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14527
  • Protein Name
    Helicase-like transcription factor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HLTF using anti-HLTF antibody (PB10070). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLTF antigen affinity purified polyclonal antibody (Catalog # PB10070) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLTF at approximately 114 kDa. The expected band size for HLTF is at 114 kDa.
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