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ProductsBack/SMARCA3 / HLTF Antibody

SMARCA3 / HLTF Antibody

LS-C403209
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHLTF
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SMARCA3 / HLTF Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6596
  • Target name
    HLTF
  • Target description
    helicase like transcription factor
  • Target synonyms
    HIP116, HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3, ZBU1, helicase-like transcription factor, DNA-binding protein/plasminogen activator inhibitor-1 regulator, RING finger protein 80, RING-type E3 ubiquitin transferase HLTF, SNF2-like 3, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, sucrose nonfermenting protein 2-like 3, sucrose nonfermenting-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HLTF using anti-HLTF antibody (PB10070). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLTF antigen affinity purified polyclonal antibody (Catalog # PB10070) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLTF at approximately 114 kDa. The expected band size for HLTF is at 114 kDa.
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