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ProductsBack/Anti-HNF1 beta Rabbit Monoclonal Antibody
Western blot analysis of HNF1 beta expression in Human kidney lysate.
Western blot analysis of HNF1 beta expression in Human kidney lysate.
Western blot analysis of HNF1 beta expression in Human kidney lysate.

Anti-HNF1 beta Rabbit Monoclonal Antibody

M01270
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
TargetHNF1B
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-HNF1 beta Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HOH-8
  • Gene ID6928
  • Target name
    HNF1B
  • Target description
    HNF1 homeobox B
  • Target synonyms
    ADTKD3, FJHN, HNF-1-beta, HNF-1B, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, RCAD, T2D, TCF-2, TCF2, VHNF1, hepatocyte nuclear factor 1-beta, HNF1 beta A, homeoprotein LFB3, transcription factor 2, hepatic
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35680
  • Protein Name
    Hepatocyte nuclear factor 1-beta
  • Scientific Description
    Boster Bio Anti-HNF1 beta Rabbit Monoclonal Antibody catalog # M01270. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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Figure 1. Western blot analysis of HNF1 beta using anti-HNF1 beta antibody (PB9597). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF1 beta antigen affinity purified polyclonal antibody (Catalog # PB9597) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF1 beta at approximately 61 kDa. The expected band size for HNF1 beta is at 61 kDa.
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