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ProductsBack/Anti-hnRNP A2/B1 [GLC82C238 ]

Anti-hnRNP A2/B1 [GLC82C238 ]

Ab03243-1.1
Absolute Antibody
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetHNRNPA2B1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-hnRNP A2/B1 [GLC82C238 ]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody was used in the immunohistochemical analysis of hnRNP A2/B1 expressed in lung squamous cell carcinoma, alveolar cancer and lung adenocarcinoma tissues. The binding of this antibody to HnRNP A2/B1 antigen was confirmed using western blotting (CN1273489).
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GLC82C238
  • Gene ID3181
  • Target name
    HNRNPA2B1
  • Target description
    heterogeneous nuclear ribonucleoprotein A2/B1
  • Target synonyms
    HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, IBMPFD2, OPMD2, RNPA2, SNRPB1, heterogeneous nuclear ribonucleoproteins A2/B1, HNRNPA2B1/MYC fusion, epididymis secretory sperm binding protein, hnRNP A2 / hnRNP B1, nuclear ribonucleoprotein particle A2 protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP22626
  • Protein Name
    Heterogeneous nuclear ribonucleoproteins A2/B1
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing BALB/ mice in the abdominal cavity with human lung cancer cell line GLC82.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP A2B1 antigen affinity purified polyclonal antibody (Catalog # A00396-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP A2B1 at approximately 37 kDa. The expected band size for hnRNP A2B1 is at 37 kDa.
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