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Anti-HnRNP F/HNRNPF Antibody

ORB2616694
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetHNRNPF
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-HnRNP F/HNRNPF Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.1-0.5microg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1microg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1microg/ml, Human Immunocytochemistry/Immunofluorescence, 2microg/ml, Human Flow Cytometry (Fixed), 1-3microg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    FC, ICC, IF, IHC, IHC-Fr, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Cy3
  • Gene ID3185
  • Target name
    HNRNPF
  • Target description
    heterogeneous nuclear ribonucleoprotein F
  • Target synonyms
    heterogeneous nuclear ribonucleoprotein F; HnRNP F protein; HNRPF; mcs94-1; nucleolin-like protein mcs94-1; OK/SW-cl.23
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52597
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein F
  • Scientific Description
    Anti-HnRNP F/HNRNPF Antibody
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HnRNPF using anti-HnRNPF antibody (A05806). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SKOV3 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human U20S whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HnRNPF antigen affinity purified polyclonal antibody (Catalog # A05806) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HnRNPF at approximately 46 kDa. The expected band size for HnRNPF is at 46 kDa.
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