![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] (GTX114476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] (GTX114476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114476/GTX114476_44524_20220121_WB_w_23060501_792.webp)
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] (GTX114476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
hnRNP F antibody [N1N3]
GTX114476
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetHNRNPF
Overview
- SupplierGeneTex
- Product NamehnRNP F antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.45 mg/ml
- ConjugateUnconjugated
- Gene ID3185
- Target nameHNRNPF
- Target descriptionheterogeneous nuclear ribonucleoprotein F
- Target synonymsHNRPF, OK/SW-cl.23, mcs94-1, heterogeneous nuclear ribonucleoprotein F, HnRNP F protein, nucleolin-like protein mcs94-1
- HostRabbit
- IsotypeIgG
- Protein IDP52597
- Protein NameHeterogeneous nuclear ribonucleoprotein F
- Scientific DescriptionThis gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012Read this paper
![HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] (GTX114476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] (GTX114476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114476/GTX114476_44524_20220211_WB_Fraction_w_23060501_246.webp)
![hnRNP F antibody [N1N3] detects hnRNP F protein at nucleus on mouse uterus by immunohistochemical analysis. Sample: Paraffin-embedded mouse uterus. hnRNP F antibody [N1N3] (GTX114476) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min hnRNP F antibody [N1N3] detects hnRNP F protein at nucleus on mouse uterus by immunohistochemical analysis. Sample: Paraffin-embedded mouse uterus. hnRNP F antibody [N1N3] (GTX114476) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX114476/GTX114476_40135_20150313_IHC_M_w_23060501_567.webp)


![Immunoprecipitation of hnRNP F protein from 293T whole cell extracts using 5 μg of hnRNP F antibody [N1N3] (GTX114476). Western blot analysis was performed using hnRNP F antibody [N1N3] (GTX114476). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. Immunoprecipitation of hnRNP F protein from 293T whole cell extracts using 5 μg of hnRNP F antibody [N1N3] (GTX114476). Western blot analysis was performed using hnRNP F antibody [N1N3] (GTX114476). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114476/GTX114476_40135_20150420_IP_w_23060501_182.webp)





![IHC-P analysis of human ovary adenocarcinoma tissue using GTX84356 hnRNP F antibody [5F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84356/GTX84356_2646_IHC-P_w_23061420_196.webp)
