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Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Anti-HOXA4 Antibody

HPA060088
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHOXA4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HOXA4 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3201
  • Target name
    HOXA4
  • Target description
    homeobox A4
  • Target synonyms
    HOX1, HOX1D, homeobox protein Hox-A4, Dfd-like protein, Hox-1.4-like protein, homeo box A4, homeobox protein Hox-1.4, homeobox protein Hox-1D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00056
  • Protein Name
    Homeobox protein Hox-A4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of HOXA4 using anti-HOXA4 antibody (A07948). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA4 antigen affinity purified polyclonal antibody (Catalog # A07948) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA4 at approximately 36 kDa. The expected band size for HOXA4 is at 36 kDa.
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