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Western Blot Positive WB detected in: Rat kidney tissue All lanes: HOXA4 antibody at 2.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: HOXA4 antibody at 2.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: HOXA4 antibody at 2.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa

HOXA4 Antibody

CSB-PA010654LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetHOXA4
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Overview

  • Supplier
    Cusabio
  • Product Name
    HOXA4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3201
  • Target name
    HOXA4
  • Target description
    homeobox A4
  • Target synonyms
    HOX1, HOX1D, homeobox protein Hox-A4, Dfd-like protein, Hox-1.4-like protein, homeo box A4, homeobox protein Hox-1.4, homeobox protein Hox-1D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00056
  • Protein Name
    Homeobox protein Hox-A4
  • Scientific Description
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5-TAATGA[CG]-3 and 5-CTAATTTT-3.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HOXA4 using anti-HOXA4 antibody (A07948). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA4 antigen affinity purified polyclonal antibody (Catalog # A07948) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA4 at approximately 36 kDa. The expected band size for HOXA4 is at 36 kDa.
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