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Immunofluorescent staining of human cell line U2OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U2OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U2OS shows localization to nucleoplasm.

Anti-HOXB1 Antibody

HPA036648
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHOXB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HOXB1 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3211
  • Target name
    HOXB1
  • Target description
    homeobox B1
  • Target synonyms
    HCFP3, HOX2, HOX2I, Hox-2.9, homeobox protein Hox-B1, homeobox protein Hox-2I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14653
  • Protein Name
    Homeobox protein Hox-B1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HOXB1 using anti-HOXB1 antibody (A04724-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate,Lane 2: mouse testis tissue lysate,Lane 3: human MCF-7 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXB1 antigen affinity purified polyclonal antibody (Catalog # A04724-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXB1 at approximately 32KD. The expected band size for HOXB1 is at 32KD.
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