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HOXB1 Antibody

LS-C400795
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHOXB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HOXB1 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:40 - 1:150)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3211
  • Target name
    HOXB1
  • Target description
    homeobox B1
  • Target synonyms
    HCFP3; homeobox protein Hox-2I; homeobox protein Hox-B1; HOX2; Hox-2.9; HOX2I
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HOXB1 using anti-HOXB1 antibody (A04724-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate,Lane 2: mouse testis tissue lysate,Lane 3: human MCF-7 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXB1 antigen affinity purified polyclonal antibody (Catalog # A04724-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXB1 at approximately 32KD. The expected band size for HOXB1 is at 32KD.
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