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Immunohistochemical staining of human liver shows moderate cytoplsmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplsmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplsmic positivity in hepatocytes.

Anti-HPX Antibody

HPA065318
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHPX
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HPX
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3263
  • Target name
    HPX
  • Target description
    hemopexin
  • Target synonyms
    beta-1B-glycoprotein; epididymis secretory sperm binding protein; hemopexin; HX
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02790
  • Protein Name
    Hemopexin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA14619A0Rb at dilution of 1:100
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Figure 1. Western blot analysis of Hemopexin/HPX using anti-Hemopexin/HPX antibody (A02237-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human placenta tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemopexin/HPX antigen affinity purified polyclonal antibody (Catalog # A02237-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hemopexin/HPX at approximately 72 kDa. The expected band size for Hemopexin/HPX is at 72 kDa.
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Atlas Antibodies
PrEST Antigen HPXAPREST94911
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