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Hemopexin Recombinant Antibody, AbBy Fluor-350 Conjugated

BSM-61636R-BF350
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetHPX
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Hemopexin Recombinant Antibody, AbBy Fluor-350 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB(1:300-5000), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID3263
  • Target name
    HPX
  • Target description
    hemopexin
  • Target synonyms
    beta-1B-glycoprotein; epididymis secretory sperm binding protein; hemopexin; HX
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02790
  • Protein Name
    Hemopexin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hemopexin/HPX using anti-Hemopexin/HPX antibody (A02237-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human placenta tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemopexin/HPX antigen affinity purified polyclonal antibody (Catalog # A02237-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hemopexin/HPX at approximately 72 kDa. The expected band size for Hemopexin/HPX is at 72 kDa.
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