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Immunofluorescent staining of human cell line Hep G2 shows localization to lipid droplets.
Immunofluorescent staining of human cell line Hep G2 shows localization to lipid droplets.
Immunofluorescent staining of human cell line Hep G2 shows localization to lipid droplets.

Anti-HSD3B7 Antibody

HPA060847
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHSD3B7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSD3B7 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80270
  • Target name
    HSD3B7
  • Target description
    hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
  • Target synonyms
    CBAS1, PFIC4, SDR11E3, 3 beta-hydroxysteroid dehydrogenase type 7, 3 beta-hydroxy-delta 5-C27-steroid oxidoreductase, 3 beta-hydroxysteroid dehydrogenase type VII, 3-beta-HSD VII, 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase, C(27)-3BETA-HSD, c(27) 3-beta-HSD, cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase, short chain dehydrogenase/reductase family 11E, member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H2F3
  • Protein Name
    3 beta-hydroxysteroid dehydrogenase type 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of HSD3B7 using anti-HSD3B7 antibody (A10025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD3B7 antigen affinity purified polyclonal antibody (Catalog # A10025-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD3B7 at approximately 45 kDa. The expected band size for HSD3B7 is at 41 kDa.
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