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Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: HSD3B7 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 22 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: HSD3B7 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 22 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: HSD3B7 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 22 kDa Observed band size: 42 kDa

HSD3B7 Antibody

CSB-PA861999LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetHSD3B7
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSD3B7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80270
  • Target name
    HSD3B7
  • Target description
    hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
  • Target synonyms
    CBAS1, PFIC4, SDR11E3, 3 beta-hydroxysteroid dehydrogenase type 7, 3 beta-hydroxy-delta 5-C27-steroid oxidoreductase, 3 beta-hydroxysteroid dehydrogenase type VII, 3-beta-HSD VII, 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase, C(27)-3BETA-HSD, c(27) 3-beta-HSD, cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase, short chain dehydrogenase/reductase family 11E, member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H2F3
  • Protein Name
    3 beta-hydroxysteroid dehydrogenase type 7
  • Scientific Description
    The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis (PubMed:11067870). Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSD3B7 using anti-HSD3B7 antibody (A10025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD3B7 antigen affinity purified polyclonal antibody (Catalog # A10025-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD3B7 at approximately 45 kDa. The expected band size for HSD3B7 is at 41 kDa.
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