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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.

Anti-HSF4 Antibody

HPA048584
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHSF4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSF4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3299
  • Target name
    HSF4
  • Target description
    heat shock transcription factor 4
  • Target synonyms
    CTM, CTRCT5, heat shock factor protein 4, HSF 4, HSTF 4, hHSF4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULV5
  • Protein Name
    Heat shock factor protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HSF4 using anti-HSF4 antibody (A04440). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF4 antigen affinity purified polyclonal antibody (Catalog # A04440) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF4 at approximately 53 kDa. The expected band size for HSF4 is at 53 kDa.
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