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ProductsBack/HSF4 antibody [2E7]
ELISA analysis of antigen using GTX60581 HSF4 antibody [2E7]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
ELISA analysis of antigen using GTX60581 HSF4 antibody [2E7]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
ELISA analysis of antigen using GTX60581 HSF4 antibody [2E7]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng

HSF4 antibody [2E7]

GTX60581
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetHSF4
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Overview

  • Supplier
    GeneTex
  • Product Name
    HSF4 antibody [2E7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3299
  • Target name
    HSF4
  • Target description
    heat shock transcription factor 4
  • Target synonyms
    CTM; CTRCT5; heat shock factor protein 4; hHSF4; HSF 4; HSTF 4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9ULV5
  • Protein Name
    Heat shock factor protein 4
  • Scientific Description
    Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HSF4 using anti-HSF4 antibody (A04440). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF4 antigen affinity purified polyclonal antibody (Catalog # A04440) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF4 at approximately 53 kDa. The expected band size for HSF4 is at 53 kDa.
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