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Anti-HSPA1L Antibody

144-01856
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetHSPA1L
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-HSPA1L Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3305
  • Target name
    HSPA1L
  • Target description
    heat shock protein family A (Hsp70) member 1 like
  • Target synonyms
    epididymis secretory sperm binding protein; heat shock 10kDa protein 1-like; heat shock 70 kDa protein 1-Hom; heat shock 70 kDa protein 1L; heat shock 70 kDa protein 1-like; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; HSP70-1L; HSP70-HOM; HSP70T; hum70t
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP34931
  • Protein Name
    Heat shock 70 kDa protein 1-like
  • Scientific Description
    HSPA1L Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HSPA1L using anti-HSPA1L antibody (A04286-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA1L antigen affinity purified polyclonal antibody (Catalog # A04286-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA1L at approximately 70 kDa. The expected band size for HSPA1L is at 70 kDa.
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