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Immunohistochemical staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemical staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemical staining of human prostate shows no positivity in glandular cells as expected.

Anti-HSPA1L Antibody

HPA043285
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHSPA1L
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSPA1L
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3305
  • Target name
    HSPA1L
  • Target description
    heat shock protein family A (Hsp70) member 1 like
  • Target synonyms
    epididymis secretory sperm binding protein; heat shock 10kDa protein 1-like; heat shock 70 kDa protein 1-Hom; heat shock 70 kDa protein 1L; heat shock 70 kDa protein 1-like; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; HSP70-1L; HSP70-HOM; HSP70T; hum70t
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP34931
  • Protein Name
    Heat shock 70 kDa protein 1-like
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, L02 whole cell lysate, 293 whole cell lysate, Raji whole cell lysate All lanes: HSPA1L antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 71 kDa Observed band size: 71 kDa
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Figure 1. Western blot analysis of HSPA1L using anti-HSPA1L antibody (A04286-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA1L antigen affinity purified polyclonal antibody (Catalog # A04286-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA1L at approximately 70 kDa. The expected band size for HSPA1L is at 70 kDa.
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