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Immunohistochemical staining of human Testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human Testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human Testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Anti-HSPA6 Antibody

HPA028549
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHSPA6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSPA6
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3310
  • Target name
    HSPA6
  • Target description
    heat shock protein family A (Hsp70) member 6
  • Target synonyms
    epididymis secretory sperm binding protein; heat shock 70 kDa protein 6; heat shock 70 kDa protein B'; heat shock 70kD protein 6 (HSP70B'); heat shock 70kDa protein 6 (HSP70B'); HSP70B'
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17066
  • Protein Name
    Heat shock 70 kDa protein 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: HSPA6 antibody at 2microg/ml Lane 1: MCF-7 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa
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Figure 1. Western blot analysis of HSPA6 using anti-HSPA6 antibody (A05402-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat smooth muscle tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse smooth muscle tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA6 antigen affinity purified polyclonal antibody (Catalog # A05402-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA6 at approximately 71 kDa. The expected band size for HSPA6 is at 71 kDa.
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Anti-HSPA6 Antibody Picoband(r)A05402-3-CARRIER-FREE
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