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Anti-HSPB2 Antibody

144-02350
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetHSPB2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-HSPB2 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3316
  • Target name
    HSPB2
  • Target description
    heat shock protein family B (small) member 2
  • Target synonyms
    HSP27, Hs.78846, LOH11CR1K, MKBP, heat shock protein beta-2, DMPK-binding protein, heat shock 27kDa protein 2, heat shock protein family B member 2, heat-shock protein beta-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16082
  • Protein Name
    Heat shock protein beta-2
  • Scientific Description
    HSPB2 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HSPB2 using anti-HSPB2 antibody (PB9598). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB2 antigen affinity purified polyclonal antibody (Catalog # PB9598) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB2 at approximately 20 kDa. The expected band size for HSPB2 is at 20 kDa.
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