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Western Blot analysis of HeLa cells using HSP beta2 Polyclonal Antibody
Western Blot analysis of HeLa cells using HSP beta2 Polyclonal Antibody
Western Blot analysis of HeLa cells using HSP beta2 Polyclonal Antibody

HSPB2 Antibody

CSB-PA002983
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSPB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSPB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3316
  • Target name
    HSPB2
  • Target description
    heat shock protein family B (small) member 2
  • Target synonyms
    HSP27, Hs.78846, LOH11CR1K, MKBP, heat shock protein beta-2, DMPK-binding protein, heat shock 27kDa protein 2, heat shock protein family B member 2, heat-shock protein beta-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16082
  • Protein Name
    Heat shock protein beta-2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSPB2 using anti-HSPB2 antibody (PB9598). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB2 antigen affinity purified polyclonal antibody (Catalog # PB9598) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB2 at approximately 20 kDa. The expected band size for HSPB2 is at 20 kDa.
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