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Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.

Anti-HTRA3 Antibody

HPA063810
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHTRA3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HTRA3
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID94031
  • Target name
    HTRA3
  • Target description
    HtrA serine peptidase 3
  • Target synonyms
    Prsp, Tasp, serine protease HTRA3, high-temperature requirement factor A3, pregnancy-related serine protease, probable serine protease HTRA3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP83110
  • Protein Name
    Serine protease HTRA3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of HtrA3 using anti-HtrA3 antibody (A05478). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: PANC-1 whole Cell lysates, Lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HtrA3 antigen affinity purified polyclonal antibody (Catalog # A05478) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HtrA3 at approximately 49KD. The expected band size for HtrA3 is at 49KD.
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