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Anti-human apoE mAb (E276), unconjugated

Anti-human apoE mAb (E276), unconjugated

3712-3
Mabtech
ApplicationsWestern Blot, ELISA, ELISpot Assay
Product group Antibodies
TargetAPOE
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human apoE mAb (E276), unconjugated
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    E276
  • Conjugate
    Unconjugated
  • Gene ID348
  • Target name
    APOE
  • Target description
    apolipoprotein E
  • Target synonyms
    AD2, APO-E, ApoE4, LDLCQ5, LPG, apolipoprotein E, apolipoprotein E3
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of human apoE in immunoassays such as ELISA, ELISpot and Western blot. The antibody is also suitable for immunoprecipitation.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APOE using anti-APOE antibody (A00015-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOE antigen affinity purified polyclonal antibody (Catalog # A00015-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOE at approximately 36 kDa. The expected band size for APOE is at 36 kDa.
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