1. Flow Cytometry analysis of A549 cells using anti-Human Bax antibody (A00183-Dyl488). Overlay histogram showing A549 cells stained with A00183-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Bax Antibody (A00183-Dyl488,1microg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
1. Flow Cytometry analysis of A549 cells using anti-Human Bax antibody (A00183-Dyl488). Overlay histogram showing A549 cells stained with A00183-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Bax Antibody (A00183-Dyl488,1microg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Anti-Human Bax DyLight(r) 488 conjugated Antibody
A00183-Dyl488
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetBAX
Overview
- SupplierBoster Bio
- Product NameAnti-Human Bax DyLight 488 conjugated Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5-1 mg/ml
- ConjugateDyLight 488
- Gene ID581
- Target nameBAX
- Target descriptionBCL2 associated X, apoptosis regulator
- Target synonymsapoptosis regulator BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2-associated X protein omega; BCL2L4; bcl2-L-4; bcl-2-like protein 4
- HostRabbit
- IsotypeIgG
- Protein IDQ07812
- Protein NameApoptosis regulator BAX
- Scientific DescriptionBoster Bio Anti-Human Bax DyLight® 488 conjugated Antibody catalog # A00183-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203