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ProductsBack/Anti-Human CD133 Antibody, RayBright(r) Blue 488

Anti-Human CD133 Antibody, RayBright(r) Blue 488

136-14095
RayBiotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetPROM1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Human CD133 Antibody, RayBright(r) Blue 488
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HB#7
  • Concentration
    5 ul/test
  • Conjugate
    Other Conjugate
  • Gene ID8842
  • Target name
    PROM1
  • Target description
    prominin 1
  • Target synonyms
    AC133; antigen AC133; CD133; CORD12; hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061; prominin-1; prominin-like protein 1; PROML1; RP41; STGD4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO43490
  • Protein Name
    Prominin-1
  • Scientific Description
    Human CD133 Antibody [clone: HB#7], monoclonal mouse IgG1. Validated for flow cytometry.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PROM1 using anti-PROM1 antibody (A01767-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROM1 antigen affinity purified polyclonal antibody (Catalog # A01767-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROM1 at approximately 97KD. The expected band size for PROM1 is at 97KD.
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