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Western Blot analysis of A549 SW620 HT29 Jurkat mouse kidney mouse lung cells using CD133 Polyclonal Antibody
Western Blot analysis of A549 SW620 HT29 Jurkat mouse kidney mouse lung cells using CD133 Polyclonal Antibody
Western Blot analysis of A549 SW620 HT29 Jurkat mouse kidney mouse lung cells using CD133 Polyclonal Antibody

PROM1 Antibody

CSB-PA005110
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPROM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PROM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8842
  • Target name
    PROM1
  • Target description
    prominin 1
  • Target synonyms
    AC133; antigen AC133; CD133; CORD12; hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061; prominin-1; prominin-like protein 1; PROML1; RP41; STGD4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43490
  • Protein Name
    Prominin-1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PROM1 using anti-PROM1 antibody (A01767-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROM1 antigen affinity purified polyclonal antibody (Catalog # A01767-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROM1 at approximately 97KD. The expected band size for PROM1 is at 97KD.
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Immunohistochemical staining of human Fallopian tube shows strong positivity in cilia in glandular cells.
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