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Western Blot analysis of A549 SW620 HT29 Jurkat mouse kidney mouse lung cells using CD133 Polyclonal Antibody
Western Blot analysis of A549 SW620 HT29 Jurkat mouse kidney mouse lung cells using CD133 Polyclonal Antibody
Western Blot analysis of A549 SW620 HT29 Jurkat mouse kidney mouse lung cells using CD133 Polyclonal Antibody

PROM1 Antibody

CSB-PA005110
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetPROM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PROM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8842
  • Target name
    PROM1
  • Target description
    prominin 1
  • Target synonyms
    AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4, prominin-1, AC133 antigen, Retinitis pigmentosa 41, Cone-rod dystrophy 12, antigen AC133, hProminin, hematopoietic stem cell antigen, prominin-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43490
  • Protein Name
    Prominin-1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PROM1 using anti-PROM1 antibody (A01767-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROM1 antigen affinity purified polyclonal antibody (Catalog # A01767-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROM1 at approximately 97KD. The expected band size for PROM1 is at 97KD.
Product group Antibodies
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Anti-PROM1 Antibody Picoband(r)A01767-3-CARRIER-FREE
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TargetPROM1
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Product group Antibodies
RayBiotech
Anti-Human CD133 Antibody, RayBright(r) Blue 488136-14095
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TargetPROM1
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Immunohistochemical staining of human Fallopian tube shows strong positivity in cilia in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-PROM1 AntibodyAMAB91494
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPROM1
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-CD133 [CD133-scFv-1]AB01481-10.0-BT
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LifeSpan BioSciences
PROM1 / CD133 AntibodyLS-C835634
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TargetPROM1
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MedChem Express
CD133 Antibody (YA808, Mouse)HY-P80054
TargetPROM1
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Everest Biotech
Goat anti-PROM1 / CD133EB11821
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TargetPROM1
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LoVo lysates probed with CD133 Polyclonal Antibody, Unconjugated (bs-4770R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
CD133 Polyclonal AntibodyBS-4770R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [C1C2], Internal (GTX100567) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
CD133 antibody [C1C2], InternalGTX100567
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetPROM1
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