More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-human CD28 mAb (CD28-A), azide free
Anti-human CD28 mAb (CD28-A), azide free

Anti-human CD28 mAb (CD28-A), azide free

3608-1
Mabtech
ReactivityHuman, Primate
Product group Antibodies
TargetCD28
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human CD28 mAb (CD28-A), azide free
  • Delivery Days Customer
    5
  • Applications Supplier
    Cell stimulation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CD28-A
  • Conjugate
    Unconjugated
  • Gene ID940
  • Target name
    CD28
  • Target description
    CD28 molecule
  • Target synonyms
    IMD123, Tp44, T-cell-specific surface glycoprotein CD28
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables co-stimulation of human T cells.
  • Reactivity
    Human, Primate
  • Reactivity Supplier
    human, non-human primates
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
Purified Anti-Human CD28 Antibody [CD28.2]AGEL2369
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
ReactivityHuman
TargetCD28
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-CD28 [YTH 913.12]Ab00195-10.0
ApplicationsFlow Cytometry, Other Application
ReactivityHuman
TargetCD28
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CD28 Antibody188-10033
ReactivityHuman
TargetCD28
  • SizePrice
Product group Antibodies
References
BPS Bioscience
Anti-CD28 Agonist Antibody100182
TargetCD28
  • SizePrice
Product group Antibodies
Sino Biological
Anti-CD28 AntibodyGMP-11524-H001
ApplicationsFunctional Assay, Flow Cytometry
ReactivityHuman
  • SizePrice
Figure 1. Western blot analysis of CD28 using anti-CD28 antibody (A00065-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HUT-78 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD28 antigen affinity purified polyclonal antibody (Catalog # A00065-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD28 at approximately 60 kDa. The expected band size for CD28 is at 25 kDa.
Product group Antibodies
Boster Bio
Anti-CD28 Antibody Picoband(r)A00065-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetCD28
  • SizePrice
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD28 antibody [HL1589] (GTX637068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
CD28 antibody [HL1589]GTX637068
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Feline, Human
TargetCD28
  • SizePrice
Product group Antibodies
Biomatik
Anti-Cluster Of Differentiation 28 (CD28) Monoclonal AntibodyCAU35828
ApplicationsFlow Cytometry
TargetCD28
  • SizePrice
Mouse spleen probed with Rabbit Anti-CD28 Polyclonal Antibody Unconjugated (bs-8865R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.\n
Product group Antibodies
References
Bioss
CD28 Polyclonal AntibodyBS-8865R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCD28
  • SizePrice
Anti-CD28 Antibody
Product group Antibodies
Antibodies.com
Anti-CD28 AntibodyA83543
ApplicationsWestern Blot, ELISA
ReactivityMouse
  • SizePrice